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PromoCell
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Cell Applications Inc
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Coriell Institute for Medical Research
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Image Search Results
Journal: Angiogenesis
Article Title: PHD2 deletion in endothelial or arterial smooth muscle cells reveals vascular cell type-specific responses in pulmonary hypertension and fibrosis.
doi: 10.1007/s10456-021-09828-z
Figure Lengend Snippet: Fig. 2 Cardiac hypertrophy, elevated pulmonary arterial and right ventricular pressures in Phd2∆ECi mice. A Representa- tive images of 10-month-old mice hearts (top) indicat- ing increased cardiac size in Phd2ΔECi. Masson trichrome staining of transverse histo- logical sections (bottom) show increased thickness of right ventricular (RV) wall (asterisk) without evidence of fibrosis or ventricle dilation. LV, left ventricle. B Increased heart weight to body weight ratio in 3 to 10 month-old Phd2ΔECi mice, n = 8–19 mice/group. C, RV wall thickness measured by M-mode in echocardiography at 3 months of age. D Pulmonary artery (PA) pressure calculated from Doppler ultrasound data. E RV developed pressure. F Rate of RV pressure develop- ment. G qPCR analysis of endothelial PAH marker genes in mouse lungs, n = 5–8 mice/ group. H qPCR analysis of endothelial PAH marker genes in ECs cultured in normoxic or hypoxic conditions for 7 days. C-F, 6–10 mice/group; H the experiments were repeated 3 to 4 times. Mean ± SD. *P < 0.05, **P < 0.01 and ***P < 0.001 in t-test. Mo, month
Article Snippet: Human ECs (PromoCell, C0155C) were cultured in
Techniques: Staining, Marker, Cell Culture